| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt East High School | A | Williams | Franklin | Bailey | Wilson | 47.28 | Verified |
| 4 | Christian High School | A | Connell | Schroer | Evans | Tucker | 46.62 | Verified |
| 5 | Ft. Zumwalt South High School | A | Pilger | Moore | Schaefering | Butler | 47.06 | Verified |
| 6 | Northwest (Cedar Hill) High Scho | A | Robinson | Sullivan | Brandt | Bounds | 48.72 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles West High School | A | Colyer | Goellner | Kirt | Scruggs | 46.16 | Verified |
| 2 | Francis Howell North High School | A | Vob Orb | Eiland | Fifer | Dowdy | 45.29 | Verified |
| 3 | Timberland High School | A | Schaefer | Whitehead | Hester | Busateri | 44.48 | Verified |
| 4 | Ft. Zumwalt North High School | A | Dickerson | Henderson | Lee | Reckel | 43.13 | Verified |
| 5 | Troy Buchanan High School | A | Hopmann | Benhardt | Matech-Edwards | Freeman | 44.13 | Verified |
| 6 | Francis Howell Central High Scho | A | Brown | Washington | Thomas | Glacken | 45.05 | Verified |
| 7 | Warrenton High School | A | Meine | Brosenne | Evans | Reel | 45.60 | Verified |
| 8 | Ft. Zumwalt West High School | A | Petty | Smith | Dreyer | Stickels | 46.43 | Verified |
