| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Francis Howell North High School | A | Huff | Huff | Bland | Murray | NT | Unverified: |
| 4 | Ft. Zumwalt West High School | A | Loggins | Anderson | St. Jean | Walden | 56.32 | Verified |
| 5 | Christian High School | A | Cunningham | Phillips | Malone | Kassebaum | 58.84 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | 54.24 | Verified | ||||
| 2 | St. Charles West High School | A | Nichols | Myers | House | Myers | 53.12 | Verified |
| 3 | Ft. Zumwalt South High School | A | Thomas | Clark | Douglas | Burnett | 51.60 | Verified |
| 4 | Timberland High School | A | Adams | Tucker | Lightbody | Utley | 50.75 | Verified |
| 5 | Troy Buchanan High School | A | Crouch | Hunolt | Starkey | Robinson | 51.59 | Verified |
| 6 | Francis Howell Central High Scho | A | Anthony | Sanders | Mabray | Johnson | 52.73 | Verified |
| 7 | Ft. Zumwalt North High School | A | Kimble | Holloway | Michel | Ramadan | 54.00 | Verified |
| 8 | Warrenton High School | A | Schleuter | Pruitt | Love | Rhama | 54.40 | Verified |
