| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles West High School | A | Jackson | Zeier | Jones | Jackson | 10:28.89 | Verified |
| 2 | Ft. Zumwalt West High School | A | Linville | Snyder | McGrath | Maupin | 10:29.68 | Verified |
| 3 | Ft. Zumwalt South High School | A | Morrison | Pawlik | Hart | Rakowski | 10:54.69 | Verified |
| 4 | Warrenton High School | A | Marschel | Morris | Howard | Marschel | 11:26.53 | Verified |
| 5 | Ft. Zumwalt North High School | A | Ebert | Bredenkamp | Anderson | Vandemark | 11:35.76 | Verified |
| 6 | Northwest (Cedar Hill) High Scho | A | 12:16.00 | Unverified: |
