| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt East High School | A | James | Franklin | Rudd | Williams | 1:43.03 | Verified |
| 2 | Ft. Zumwalt West High School | A | Carr | Eley | Smith | Stickels | 1:37.00 | Verified |
| 3 | Ft. Zumwalt South High School | A | Bates | Powell | Raife | Dewitt | 1:36.47 | Verified |
| 4 | Francis Howell Central High Scho | A | Thomas | Washington | Brown | Humphries | 1:34.78 | Verified |
| 5 | Troy Buchanan High School | A | Benhardt | Giacopelli | Freeman | Ryan | DQ | Verified |
| 6 | St. Charles West High School | A | Goellner | Colyer | Hunsperger | Politte | DQ | Verified |
| 7 | St. Charles High School | A | Calmese | Newberry | Thompson | Chang | 1:39.49 | Verified |
