| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles High School | A | Walker | Hollrah | Oetting | Long | 1:59.21 | Verified |
| 2 | North Point High School | A | Anderson | Saunders | Pender | Toulou | 1:55.80 | Verified |
| 3 | Ft. Zumwalt South High School | A | Moore | Clark | Johnson | Burnett | 1:52.93 | Verified |
| 4 | Troy Buchanan High School | A | Crouch | Robinson | Starkey | Hunolt | 1:51.30 | Verified |
| 5 | Francis Howell Central High Scho | A | Anthony | Johnson | Sanders | Mabray | 1:52.03 | Verified |
| 6 | Ft. Zumwalt West High School | A | Rowland | Washington | Mueller | Rider | 1:55.12 | Verified |
| 7 | St. Charles West High School | A | Shivers | Myers | Prost | Boxdorfer | 1:58.92 | Verified |
