| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell Central High Scho | A | Eggering | Barnes | Kliewer | Mann | NT | Unverified: |
| 3 | St. Charles High School | A | Simon | Cortes-Lopez | Hoffmann | Klimas | NT | Unverified: |
| 4 | Ft. Zumwalt East High School | A | Henderson | Rowold | Albers | Vick | 57.54 | Verified |
| 5 | Ft. Zumwalt West High School | A | Dixon | Entwistle | Rowland | Lu | 58.94 | Verified |
| 6 | St. Charles West High School | A | Lee | Hudson | Govero | Buehrle | NT | Unverified: |
