| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Point High School | A | Paul | Lacke | Trennepohl | Trennepohl | 3:57.51 | Verified |
| 2 | Troy Buchanan High School | A | Benhardt | Hogan | Ryan | Ballard | 3:47.19 | Verified |
| 3 | Francis Howell Central High Scho | A | Kinion | McDevitt | Martin | Jones | 3:45.18 | Verified |
| 4 | Ft. Zumwalt West High School | A | Carr | Potocki | Dubowski | Eley | 3:40.31 | Unverified: |
| 5 | Ft. Zumwalt South High School | A | Raife | Clark | Dewitt | Bates | 3:40.57 | Verified |
| 6 | Ft. Zumwalt East High School | A | Wilmes | Patel | Borger | El Farissi | 3:47.16 | Verified |
| 7 | St. Charles High School | A | Bland | Warner | Nennert | Nottmeyer | 3:47.52 | Verified |
| 8 | St. Charles West High School | A | Goellner | Hunsperger | Colyer | Scruggs | NT | Verified |
