| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Francis Howell Central High Scho | A | Breuer | Halterman | McDevitt | Myers | 4:49.05 | Verified |
| 3 | Ft. Zumwalt South High School | A | Hart | Harper | Whitten | Pawlik | 4:38.40 | Verified |
| 4 | St. Charles West High School | A | Myers | Prost | Zeier | Jackson | 4:23.15 | Verified |
| 5 | Ft. Zumwalt West High School | A | Anderson | Mueller | Maupin | Rider | 4:29.24 | Verified |
| 6 | St. Charles High School | A | Oellermann | Long | Gilkison | O'Keeffe | 4:45.97 | Verified |
| 7 | Troy Buchanan High School | A | Cook | Ellison | Waechter | Getz | NT | Unverified: |
