| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt West High School | A | Hammond | Fanter | Whitaker | Dubowski | 8:45.06 | Unverified: |
| 2 | St. Charles West High School | A | Litton | Wortham | Spraul | Kudaravalli | 8:47.65 | Verified |
| 3 | St. Charles High School | A | Maples | Warner | Nottmeyer | Bland | 8:55.66 | Verified |
| 4 | Ft. Zumwalt East High School | A | Borger | Patel | El Farissi | Anderson | 9:01.55 | Verified |
| 5 | Francis Howell Central High Scho | A | Morris | McCracken | Woelfel | Wren | 9:11.17 | Verified |
| 6 | Ft. Zumwalt South High School | A | Bates | Moore | Bass | Walker | 9:33.81 | Verified |
| 7 | North Point High School | A | Blase | Lacke | Robinson | Whitehead | 9:47.20 | Verified |
| 8 | Troy Buchanan High School | A | Orf | Peek | Carr | Mahler | 10:05.83 | Verified |
