| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles West High School | A | Hunsperger | Kirt | Parrish | Regot | 49.57 | Verified |
| 2 | Ft. Zumwalt South High School | A | Raife | Dewitt | Butler | Powell | 47.92 | Verified |
| 3 | Ft. Zumwalt West High School | A | Carr | Jones | Petty | Smith | 46.43 | Verified |
| 4 | Troy Buchanan High School | A | Benhardt | Bova | Hogan | Matech-Edwards | 45.22 | Verified |
| 5 | Francis Howell Central High Scho | A | Washington | Thomas | Brown | Glacken | 45.45 | Verified |
| 6 | St. Charles High School | A | Chang | Murphy | Calmese | Thompson | 46.79 | Verified |
| 7 | Ft. Zumwalt East High School | A | Franklin | Williams | Bailey | Wilson | 48.57 | Verified |
