| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles West High School | A | Myers | Jones | Jackson | Jackson | 10:37.32 | Verified |
| 2 | Ft. Zumwalt West High School | A | Linville | McGrath | Snyder | Maupin | 10:40.62 | Verified |
| 3 | Ft. Zumwalt South High School | A | Hart | Pawlik | Morrison | Holman | 11:01.60 | Verified |
| 4 | St. Charles High School | A | O'Keeffe | Rayburn | Nash | Gilkison | 11:24.90 | Verified |
