| PL | Team | Squad | Time | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | ||||
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| 1 | Ft. Zumwalt West High School | A | 59.95 |
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| 2 | Ft. Zumwalt East High School | A | 1:01.55 |
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| 3 | St. Charles High School | A | 1:05.46 |
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| St. Charles West High School | A | NT |
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| Francis Howell Central High Scho | A | NT |
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