| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ELLIS, Matera | JR | Mark Twain High School | 6:01.00 | Verified |
| 2 | HAYS, Ella | SO | Monroe City High School | 6:10.65 | Verified |
| 3 | MILLER, Sydney | 9 | Mark Twain High School | 6:27.05 | Verified |
| 4 | JOHNSON, Avery | 9 | Monroe City High School | 6:35.37 | Verified |
| 5 | WEITKAMP, Macy | SR | Clopton High School | 7:32.09 | Verified |
| 6 | EGGEMEYER, Victoria | SO | Clopton High School | 7:43.34 | Verified |
