| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | Jung | Miller | Lake | Preston | 1:42.60 | Verified |
| 2 | Pittsfield High School | A | 1:37.32 | Verified | ||||
| 3 | Bowling Green High School | A | Bryant | Dalton | Gamm | Starks | 1:34.19 | Verified |
| 4 | Monroe City High School | A | Jones | Countryman | Talton | Martinez | 1:34.64 | Verified |
| 5 | Louisiana High School | A | Greene | Chatman | Washington | Richards | 1:38.62 | Verified |
| 6 | Van-Far High School | A | Baugh | Woodson | Walsh | Penley | 1:57.97 | Verified |
