| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Emmalee | SR | Monroe City High School | 2:21.60 | Verified |
| 2 | WILLIAMS, Hannah | 9 | Pittsfield High School | 2:43.89 | Verified |
| 3 | DEJAYNES, Michelle | 9 | Pittsfield High School | 2:48.00 | Unverified: |
| 4 | ELLIS, Matera | JR | Mark Twain High School | 2:53.17 | Verified |
| 5 | JOHNSON, Avery | 9 | Monroe City High School | 2:56.31 | Verified |
| 6 | BOOTH, Lauren | JR | Mark Twain High School | 2:59.05 | Verified |
| 7 | BIBB, Dianna | 9 | Bowling Green High School | 3:00.90 | Verified |
| 8 | BIBB, Claire | 9 | Bowling Green High School | 3:02.71 | Verified |
| 9 | EGGEMEYER, Victoria | SO | Clopton High School | 3:18.75 | Verified |
| 10 | INCE, Alexis | SR | Louisiana High School | 3:21.65 | Verified |
| 11 | INCE, Anna | SO | Louisiana High School | 3:33.26 | Verified |
| 12 | SMITH, Braya | SO | Clopton High School | NT | Unverified: |
