| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WELLS, Natalee | SO | Bowling Green High School | 1:00.72 | Verified |
| 2 | JAMES, Ashley | 9 | Silex High School | 56.98 | Verified |
| 3 | YOUNGBLOOD, Audri | 9 | Monroe City High School | 54.00 | Unverified: |
| 4 | FOSS, Gracee | 9 | Monroe City High School | 54.39 | Verified |
| 5 | RESOR, Cynthia | SO | Mark Twain High School | 59.74 | Verified |
| 6 | MYERS, Faith | JR | Clopton High School | 1:04.29 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, Riyeen | 9 | Bowling Green High School | 52.99 | Verified |
| 2 | SMITH, Lillian | SR | Elsberry High School | 51.70 | Verified |
| 3 | TEN EYCK, Ellie | JR | Pittsfield High School | 49.16 | Unverified: |
| 4 | COX, Katie | SR | Pittsfield High School | 50.28 | Unverified: |
| 5 | BRYAN, Alayna | SR | Van-Far High School | 52.94 | Unverified: Montgomery Invite |
| 6 | MILLER, Sydney | 9 | Mark Twain High School | 53.13 | Verified |
