| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NORTHCUTT, Sam | 9 | Mark Twain High School | 5:00.75 | Verified |
| 2 | SOTO, Jose | JR | Bowling Green High School | 5:15.07 | Verified |
| 3 | VEILE, Brayden | JR | Pittsfield High School | 5:15.07 | Verified |
| 4 | BLACK, Brandon | JR | Mark Twain High School | 5:15.57 | Verified |
| 5 | OLSON, Vinny | SO | Pittsfield High School | 5:19.27 | Verified |
| 6 | HENNINGFIELD, Elija | 9 | Bowling Green High School | 5:25.32 | Verified |
| 7 | HUNTER, Aidan | 9 | Monroe City High School | 5:48.17 | Verified |
| 8 | CHEEK, Korbyn | JR | Monroe City High School | 5:55.00 | Verified |
| 9 | HAYDEN, Richard | SO | Van-Far High School | 6:10.53 | Verified |
| 10 | CHESSER, Noah | SO | Silex High School | 9:59.40 | Verified |
| 11 | BANKHEAD, Michael | JR | Clopton High School | NT | Unverified: |
