| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bland Middle School | A | Foster | Anderson | Fredendall | Weigel | 2:04.39 | Verified |
| 2 | Cuba Middle School | A | Jones | Boatright | Carnal | Laney | 1:55.16 | Verified |
| 3 | Sullivan Middle School | A | Ray | Rochleau | Ray | Harmon | 1:49.72 | Unverified: |
| 4 | Waynesville Middle School | A | Nelson | Garland | Davis | Fielding-Chiles | 1:43.81 | Verified |
| 5 | St. Clair Junior High School | A | Bartz | Fults | Rulo | Bradshaw | 1:47.49 | Verified |
| 6 | St. James Middle School | A | Lewis | Holt | Byron | Moreland | 1:50.00 | Verified |
| 7 | Steelville Middle School | A | Johnson | Dicus | Alexander | Bruno | 2:00.66 | Verified |
| 8 | Licking Middle School | A | Corley | Lyons | Rinne | Stout | 2:10.37 | Verified |
