| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Licking Middle School | A | Gettemeyer | Lay | Mitchell | Thompson | 2:16.94 | Verified |
| 2 | Steelville Middle School | A | Pryor | Schmidt | Eilert | Decker | 2:06.18 | Verified |
| 3 | St. James Middle School | A | Roark | Snyder | Hendry | Foust | 2:04.70 | Verified |
| 4 | Waynesville Middle School | A | Harris | Bigelow | Madison | Daniely | 1:53.64 | Verified |
| 5 | Sullivan Middle School | A | Anderson | Bollinger | Harbour | Schmuke | 2:03.82 | Unverified: |
| 6 | St. Clair Junior High School | A | Shelton | Talleur | Snell | Draves | 2:06.17 | Verified |
| 7 | Cuba Middle School | A | Farris | Schmidt | Harris | Garbo | 2:11.16 | Verified |
| 8 | Vienna Middle School | A | Magner | Martin | Davis | Hollis | 2:17.39 | Verified |
