| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bland Middle School | A | Anderson | Gehlert | Fredendall | Weigel | 4:50.35 | Verified |
| 2 | Vienna Middle School | A | Schwartze | Douglas | Rosentreter | Magner | 4:39.44 | Verified |
| 3 | Sullivan Middle School | A | Dudley | Ray | Pritchett | Harmon | 4:18.58 | Verified |
| 4 | Waynesville Middle School | A | Davis | Boyd | Garland | Hamilton | 4:04.47 | Verified |
| 5 | St. Clair Junior High School | A | Bartz | Fults | Pittman | Bradshaw | 4:05.85 | Verified |
| 6 | St. James Middle School | A | Gruver | Lewis | Perkins | Siders | 4:23.20 | Verified |
| 7 | Cuba Middle School | A | Hagy | Henke | Munoz | Wheeler | 4:42.74 | Verified |
| 8 | Steelville Middle School | A | Richter | Shoemake | Gill | Dicus | 4:59.94 | Verified |
