| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bland Middle School | A | Barzee | Doss | LeRoux | Rehmert | 1:01.82 | Verified |
| 4 | Steelville Middle School | A | Johnson | Dicus | Keymann | Alexander | 57.34 | Verified |
| 5 | Vienna Middle School | A | Douglas | Crum | Rosentreter | Magner | 1:00.39 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Sullivan Middle School | A | Harmon | Ray | Ray | Pritchett | 53.39 | Verified |
| 3 | Cuba Middle School | A | Noltkamper | Laney | Boatright | Halbert | 50.52 | Verified |
| 4 | St. Clair Junior High School | A | Bartz | Fults | Rulo | Bradshaw | 50.07 | Verified |
| 5 | Waynesville Middle School | A | Nelson | Fielding-Chiles | Goins | Howard | 50.29 | Verified |
| 6 | St. James Middle School | A | Boone | Byron | Gruver | Moreland | 51.77 | Verified |
| 7 | Licking Middle School | A | Lyons | Rinne | Stout | Frazier | 56.63 | Verified |
