| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bourbon High School | A | Clonts | Poole | Gebhart | Enke | NT | Unverified: |
| 3 | Cuba High School | A | Deluna | Dong | Moreland | Gilbert | 2:22.14 | Verified |
| 4 | Owensville High School | A | Daniels | Daniels | Kohrmann | Carpenter | 1:57.89 | Unverified: Montgomery County Early Invite |
| 5 | Vienna High School | A | Novak | Reeves | George | Weeks | 1:58.60 | Unverified: Vienna |
| 6 | Newburg High School | A | Harris | Oliver | Pratt | Mace | 2:28.00 | Verified |
