| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bourbon High School | A | Pietraschke | Politte | Reeves | Reeves | NT | Unverified: |
| 3 | Cuba High School | A | Nelson | Scott | Hunter | Hinojosa | 4:10.00 | Verified |
| 4 | Owensville High School | A | Breedlove | Payne | Soest | Valley | 3:42.00 | Unverified: Montgomery County Early Invite |
| 5 | Vienna High School | A | Auten | George | Wilkinson | Kilmer | 4:04.00 | Unverified: Vienna |
| 6 | Steelville High School | A | Brown | Diaz | Killeen | Harris | NT | Unverified: |
