| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bourbon High School | A | Clonts | Gebhart | Poole | Enke | NT | Unverified: |
| 3 | Cuba High School | A | Moreland | Wools | Gilbert | Deluna | 5:33.52 | Verified |
| 4 | Steelville High School | A | Diaz | Davis | Turnbough | Pryor | 4:42.74 | Verified |
| 5 | Vienna High School | A | Schell | George | Murphy | Reeves | 4:55.00 | Unverified: Osage |
| 6 | Owensville High School | A | Kohrmann | Abell | Finn | Kandlbinder | NT | Unverified: |
