| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HAHN, Jayden | SO | Steelville High School | NT | Unverified: |
| 4 | SETZER, Boston | 9 | Steelville High School | NT | Unverified: |
| 5 | MASSEY, John | JR | Cuba High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MURPHY, Michael | 9 | Viburnum High School | 1:05.57 | Verified |
| 3 | GIBBS, Keaton | 9 | Bourbon High School | 1:01.22 | Verified |
| 4 | MARSHALL, Weston | SO | Montgomery County High School | 1:00.26 | Verified |
| 5 | DONATTI, Carson | SR | Owensville High School | 1:00.95 | Verified |
| 6 | RODGERS, Sean | 9 | Montgomery County High School | 1:03.41 | Verified |
| 7 | WELLS, Riordan | SR | Viburnum High School | 1:06.38 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KILLEEN, Kyle | JR | Steelville High School | 58.37 | Verified |
| 2 | BROWN, Toby | SR | Steelville High School | 56.07 | Verified |
| 3 | MEYER, Tanner | SO | Owensville High School | 54.55 | Verified |
| 4 | BREEDLOVE, Jacob | SR | Owensville High School | 51.17 | Verified |
| 5 | SOEST, Gabriel | JR | Owensville High School | 54.00 | Unverified: |
| 6 | VALLEY, Landon | SR | Owensville High School | 56.00 | Unverified: |
| 7 | MAGNER, Spencer | SR | Vienna High School | 56.70 | Unverified: Vienna |
| 8 | REEVES, Zackary | SO | Bourbon High School | 59.44 | Verified |
