| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Newburg High School | A | Harris | Mace | Oliver | Brinkley | NT | Unverified: |
| 3 | Vienna High School | A | Novak | Rosentreter | Schell | George | 55.60 | Unverified: Vienna |
| 4 | Steelville High School | A | Edgar | Davis | Pryor | Woodall | 54.00 | Unverified: |
| 5 | Owensville High School | A | Daniels | Daniels | Echols | Carpenter | 55.44 | Verified |
| 6 | Cuba High School | A | Deluna | Dong | Moreland | Martellaro | 1:06.25 | Verified |
