| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Steelville High School | A | Turnbough | Collinson | Diaz | Pryor | 11:14.39 | Verified |
| 2 | Newburg High School | A | Brinkley | Caquelin | Logan | Harris | 12:43.02 | Verified |
| 3 | Cuba High School | A | Brown | Bussell | Fulliam | Wools | 13:10.37 | Verified |
| 4 | Vienna High School | A | Novak | Reeves | Wieberg | Kloeppel | 13:35.00 | Unverified: Osage |
| 5 | Owensville High School | A | Abell | Limberg | Weinland | Gibson | NT | Unverified: |
