| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FULLIAM, Kaylee | SO | Cuba High School | 5:53.69 | Verified |
| 2 | CAQUELIN, Akaela | 9 | Newburg High School | 6:00.16 | Verified |
| 3 | TURNBOUGH, Audrey | SO | Steelville High School | 6:09.37 | Verified |
| 4 | PERONA, Vanessa | SO | St. James High School | 6:13.94 | Verified |
| 5 | DIAZ, Courtney | JR | Steelville High School | 6:15.31 | Unverified: Linn High School Invitational |
| 6 | COFFELT, Madelyn | JR | Crocker High School | 6:17.62 | Verified |
| 7 | BROWN, Melayna | SR | Cuba High School | 6:28.45 | Verified |
| 8 | CRONIN, Janelle | 9 | New Haven High School | 6:40.00 | Verified |
| 9 | MCDANIEL, Tajel | 9 | Belle High School | 6:42.65 | Verified |
| 10 | GRUBB, Sydney | SO | New Haven High School | 6:56.48 | Verified |
| 11 | KINKEAD, Emily | SR | St. James High School | 6:59.19 | Verified |
| 12 | MURPHY, Danielle | JR | Vienna High School | 7:46.19 | Verified |
