| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Cuba High School | A | Hinojosa | Nelson | Dake | Volner | 1:45.60 | Verified |
| 3 | Crocker High School | A | 1:41.96 | Verified | ||||
| 4 | Steelville High School | A | Brown | Brown | Harris | Killeen | 1:40.59 | Verified |
| 5 | St. Paul Lutheran (Farmington) | A | 1:40.75 | Verified | ||||
| 6 | St. James High School | A | Bullock | Altis | Gruver | Redburn | 1:42.21 | Verified |
| 8 | Viburnum High School | A | Hardy | Zundell | Murphy | Hubbs | 1:51.91 | Verified |
