| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Paul Lutheran (Farmington) | A | 4:15.93 | Verified | ||||
| 2 | Vienna High School | A | Auten | Wilkinson | Magner | Steffen | 4:03.99 | Verified |
| 3 | Dixon High School | A | Dalton | Solan | Dalton | D'mellow | 3:58.88 | Unverified: |
| 4 | Steelville High School | A | Diaz | Brown | Killeen | Harris | 3:38.89 | Verified |
| 5 | St. James High School | A | Bullock | Altis | Shields | Redburn | 3:58.78 | Verified |
| 6 | Cuba High School | A | Dake | Hinojosa | Hunter | Volner | 4:01.66 | Verified |
