| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Paul Lutheran (Farmington) | A | 5:26.66 | Verified | ||||
| 3 | New Haven High School | A | Steele | Luecke | Groner | Strubberg | 4:45.29 | Verified |
| 4 | Steelville High School | A | Davis | Diaz | Kreitner | Pryor | 4:37.21 | Verified |
| 5 | Vienna High School | A | Reeves | Schell | Weeks | Schulte | 4:44.32 | Verified |
| 6 | Cuba High School | A | Deluna | Ijames | Moreland | Wools | 5:18.86 | Verified |
