| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BLACKMON, Elissa | SO | St. Paul Lutheran (Farmington) | NT | Unverified: |
| 4 | BAER, Anna | 9 | St. Paul Lutheran (Farmington) | 36.55 | Verified |
| 5 | SOPPELAND, Tanya | 9 | Cuba High School | 38.42 | Verified |
| 6 | TODD, Kelsey | SR | Crocker High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SASFY, Jordan | 9 | Crocker High School | 33.11 | Verified |
| 2 | HALBERT, Natalie | 9 | Viburnum High School | 32.27 | Verified |
| 3 | PERKINS, Marli | SO | Steelville High School | 31.51 | Verified |
| 4 | VOGELSANG, BROOKELYN | 9 | New Haven High School | 31.14 | Verified |
| 5 | DEARDEUFF, Erika | JR | St. James High School | 31.44 | Verified |
| 6 | SETZER, Navy | JR | St. James High School | 31.68 | Verified |
| 7 | ROSENTRETER, Riley | JR | Vienna High School | 33.01 | Verified |
| 8 | GRIFFIN, Aliyah | SO | Vienna High School | 35.08 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PRATT, Ciarra | SO | Newburg High School | 31.02 | Verified |
| 2 | EDGAR, Erica | 9 | Steelville High School | 29.98 | Verified |
| 3 | BUTLER, Madeline | SR | Dixon High School | 29.82 | Verified |
| 4 | KECK, Montgomery | SO | Dixon High School | 28.33 | Unverified: Vienna Black and Gold Invitation |
| 5 | DONG, Justina | SR | Cuba High School | 29.50 | Verified |
| 6 | BARKER, Alexus | SR | Viburnum High School | 29.84 | Verified |
| 7 | HARRIS, Kaliethia | JR | Newburg High School | 30.91 | Verified |
| 8 | GRATER, Chloe | SR | New Haven High School | 31.04 | Verified |
