| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Conner | JR | Steelville High School | 2:02.76 | Verified |
| 2 | ROMO-VAZQUEZ, Jose | SO | New Haven High School | 2:22.35 | Verified |
| 4 | HENKE, Dustin | SR | Cuba High School | 2:27.12 | Verified |
| 5 | PEAK, Brett | JR | St. Paul Lutheran (Farmington) | 2:30.65 | Verified |
| 6 | DANIELSON III, Jeffrey | SO | Crocker High School | 2:31.00 | Verified |
| 7 | DALTON, Daxx | SO | Dixon High School | 2:32.07 | Verified |
| 8 | BAILEY, Curtis | SO | Steelville High School | 2:32.57 | Verified |
| 9 | KILMER, Jackson | SO | Vienna High School | 2:42.24 | Verified |
| 10 | MURPHY, Michael | 9 | Viburnum High School | 2:44.31 | Verified |
| 11 | FINNEGAN, Barrett | 9 | Belle High School | 2:49.67 | Verified |
| 12 | O'NEAL, Jordan | 9 | Dixon High School | 3:04.29 | Verified |
| 13 | MASSEY, John | JR | Cuba High School | 3:24.35 | Verified |
| 14 | ZUNDELL, Kameron | 9 | Viburnum High School | NT | Unverified: |
