| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KLOEPPEL, Ava | 9 | Vienna High School | 2:21.00 | Unverified: Osage |
| 2 | DIAZ, Courtney | JR | Steelville High School | 2:36.77 | Verified |
| 3 | CAQUELIN, Akaela | 9 | Newburg High School | 2:42.78 | Verified |
| 4 | COFFELT, Madelyn | JR | Crocker High School | 2:51.51 | Verified |
| 5 | CRONIN, Janelle | 9 | New Haven High School | 2:52.31 | Verified |
| 6 | CONAWAY, Jorja | SO | Viburnum High School | 2:57.21 | Verified |
| 7 | COLLINSON, Aleah | SO | Steelville High School | 2:58.06 | Verified |
| 8 | IJAMES, Izzabelle | SO | Cuba High School | 2:58.32 | Verified |
| 9 | CRONIN, Elaina | SO | New Haven High School | 3:13.71 | Verified |
| 10 | MURPHY, Danielle | JR | Vienna High School | 3:18.17 | Verified |
| 11 | PETERSON, April | 9 | Viburnum High School | 3:24.43 | Verified |
| 12 | VALLEY, Jessalynn | 9 | Dixon High School | 3:29.82 | Verified |
| 13 | DICKENS, Kaitlin | JR | Cuba High School | 3:42.99 | Verified |
