| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARTELLARO, Rachel | 9 | Cuba High School | 1:04.08 | Verified |
| 2 | PERKINS, Naomi | SR | Steelville High School | 1:00.00 | Unverified: |
| 3 | DAVIS, Lauren | SR | Steelville High School | 57.64 | Unverified: Union Don Olszowka Invitational |
| 4 | KECK, Montgomery | SO | Dixon High School | 51.68 | Verified |
| 5 | TWYMAN, Corra | JR | Newburg High School | 53.64 | Verified |
| 6 | WIGGER, Natalie | 9 | Viburnum High School | 58.24 | Verified |
| 7 | CONSTIEN, Sarah | 9 | St. Paul Lutheran (Farmington) | 1:00.62 | Verified |
| 8 | MAUNTEL, Kyra | SO | New Haven High School | 1:10.60 | Verified |
