| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | OLIVER, Aubrianna | 9 | Newburg High School | NT | Unverified: |
| 4 | BUSSELL, Allyson | JR | Cuba High School | 1:22.27 | Verified |
| 5 | SANTOS, Rachelle | SR | Dixon High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | PRATT, Ciarra | SO | Newburg High School | 1:18.95 | Verified |
| 3 | KINKEAD, Emily | SR | St. James High School | 1:18.13 | Verified |
| 4 | LUECKE, Elizabeth | SO | New Haven High School | 1:14.88 | Verified |
| 5 | MCCARTY, Taylor | SO | St. Paul Lutheran (Farmington) | 1:17.93 | Verified |
| 6 | NOVAK, Madeleine | JR | Vienna High School | 1:18.94 | Verified |
| 7 | VIRAMONTES, Annia | SO | Cuba High School | 1:19.89 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STRUBBERG, Avery | 9 | New Haven High School | 1:11.32 | Verified |
| 2 | TODD, Kelsey | SR | Crocker High School | 1:10.14 | Verified |
| 3 | BARKER, Alexus | SR | Viburnum High School | 1:08.05 | Verified |
| 4 | PRYOR, Daylan | JR | Steelville High School | 1:05.14 | Verified |
| 5 | WEEKS, Madison | 9 | Vienna High School | 1:06.57 | Verified |
| 6 | WIGGER, Natalie | 9 | Viburnum High School | 1:09.16 | Verified |
| 7 | BUTLER, Madeline | SR | Dixon High School | 1:11.26 | Verified |
| 8 | EVANS, Norah | SO | Steelville High School | 1:14.06 | Verified |
