| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vienna High School | A | Carnes | Casey | George | Rollins | 55.39 | Verified |
| 2 | St. Paul Lutheran (Farmington) | A | 51.99 | Verified | ||||
| 3 | Cuba High School | A | Nelson | Hinojosa | Declue | DeClue | 48.65 | Verified |
| 4 | St. James High School | A | Bullock | Altis | Gruver | Inda | 47.64 | Verified |
| 6 | Steelville High School | A | Brown | Pounds | Harris | Killeen | 49.39 | Verified |
| 7 | Dixon High School | A | Poole | D'mellow | Dalton | Vineyard | 54.39 | Unverified: |
