| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Seckman High School | A | Gossett | Harp | Tilley | Rudanovich | DQ | Verified |
| 2 | Kirkwood High School | A | Jones | Richardson | Marion | Brown | 1:30.17 | Verified |
| 3 | Parkway West High School | A | Harris | Bell | Campbell | Wayne | 1:29.41 | Verified |
| 4 | Ritenour High School | A | Kadima | Williams | Robinson | Watson | 1:28.61 | Verified |
| 5 | St. Louis University High School | A | Harris | Oliphant | Brooks | Wingo | 1:30.17 | Verified |
| 6 | Marquette High School | A | Brown | WILLIAMS | ELLIS | CODY-JACKSON | 1:29.48 | Verified |
| 7 | Poplar Bluff High School | A | McBride | mitchell | Gatewood | Hoss | 1:30.42 | Verified |
| 8 | Mehlville High School | A | Jones | Clayton | DUBOSE | OHMER | 1:35.34 | Verified |
