| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | OLIPHANT, Jacobi | SO | St. Louis University High School | 22.92 | Verified |
| 2 | HENDRICKS, Amar'e | 9 | Christian Brothers College High | 22.67 | Verified |
| 3 | MITCHELL, justin | SR | Poplar Bluff High School | 22.22 | Verified |
| 4 | WINGO, Ryan | SO | St. Louis University High School | 22.04 | Verified |
| 5 | HYKEN, Cooper | SR | John Burroughs High School | 22.43 | Verified |
| 6 | GRAY, Christian | JR | DeSmet Jesuit High School | 22.64 | Verified |
| 7 | HOSS, Kasen | SR | Poplar Bluff High School | 22.74 | Verified |
| 8 | MARION, Evan | 9 | Kirkwood High School | 23.14 | Verified |
