| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FLIEGE, Layla | SR | Jackson High School | 12:53.85 | Verified |
| 2 | KLEIN, Margaret | SR | Kirkwood High School | 12:43.62 | Verified |
| 3 | SINGLETON, Magdalena | JR | Parkway South High School | 12:20.41 | Verified |
| 4 | MARCUM, Isabella | JR | Fox High School | 12:03.73 | Verified |
| 5 | WHITAKER, Kennedy | JR | Parkway West High School | 12:08.11 | Verified |
| 6 | FINAN, Rachel | SR | Kirkwood High School | 12:40.68 | Verified |
| 7 | MENDEZ HIGUERA, Maite | SR | Jackson High School | 12:44.63 | Verified |
| 8 | PARISI, MARIA | SO | Oakville High School | 13:00.16 | Verified |
