| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WELCH, Nora | SR | Webster Groves High School | 2:27.52 | Verified |
| 2 | WAGNER, Elizabeth | SR | John Burroughs High School | 2:23.13 | Verified |
| 3 | FLIEGE, Layla | SR | Jackson High School | 2:19.64 | Verified |
| 4 | METZGER, Mallorie | JR | Jackson High School | 2:19.61 | Verified |
| 5 | BAKER, Josie | SO | Kirkwood High School | 2:22.42 | Verified |
| 6 | SCHULTE, Virginia | SR | Ladue Horton Watkins High School | 2:22.75 | Verified |
| 7 | TANZOLA, Audrey | SR | Marquette High School | 2:26.75 | Verified |
| 8 | FORBES, Taylor | SO | Lindbergh High School | 2:28.60 | Verified |
