| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCCLURE, Alli | SO | Kirkwood High School | 50.70 | Verified |
| 2 | WATTS, Cecelia | JR | Parkway West High School | 49.68 | Verified |
| 3 | TAYLOR, alexis | JR | Cardinal Ritter High School | 46.96 | Verified |
| 4 | SPAIN, Kyndall | 9 | Cardinal Ritter High School | 44.96 | Verified |
| 5 | DIXON, Genesis | JR | Kirkwood High School | 44.98 | Verified |
| 6 | MURDOCK, Samantha | SO | Webster Groves High School | 49.15 | Verified |
| 7 | SMITH, Emma | SR | Ladue Horton Watkins High School | 49.74 | Verified |
| 8 | O'SHEA, Jenna | JR | Mehlville High School | 51.13 | Verified |
