| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GRIFFIN, Karina | 9 | Ladue Horton Watkins High School | 1:03.02 | Verified |
| 2 | WARD, Angela | JR | Seckman High School | 1:00.89 | Verified |
| 3 | BRYANT, Neveah | 9 | Cardinal Ritter High School | 58.59 | Verified |
| 4 | MOODY, charlye | SO | Cardinal Ritter High School | 57.56 | Verified |
| 5 | MOTES, Kayliah | SR | Ladue Horton Watkins High School | 57.85 | Verified |
| 6 | TROUPE, JOSHLYN | JR | Marquette High School | 1:00.67 | Verified |
| 7 | CZUPPON, Addison | SO | Oakville High School | 1:01.74 | Verified |
| 8 | BURGETT, Karla | SO | Kirkwood High School | 1:04.85 | Verified |
