| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAKER, Jackson | SR | Lindbergh High School | 52.81 | Verified |
| 2 | ROBINSON, Tyson | SO | Ritenour High School | 50.87 | Verified |
| 3 | BROWN, Derrick | SR | Marquette High School | 50.15 | Verified |
| 4 | CODY-JACKSON, CALEB | JR | Marquette High School | 49.24 | Verified |
| 5 | JONES, James | SR | Webster Groves High School | 51.53 | Verified |
| 6 | WILLIAMS, Samuel | SR | Ritenour High School | 50.45 | Verified |
| 7 | RUDANOVICH, Kaleb | JR | Seckman High School | 52.52 | Verified |
| 8 | MAYER, Steffan | JR | St. Louis University High School | 52.82 | Verified |
