| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Battle High School | A | Nelson | Anderson | Lakes | Karara | 3:39.19 | Verified |
| 2 | Hickman High School | A | Zink | Keys | Holt | Abrams | 3:31.57 | Verified |
| 3 | Timberland High School | A | Mclaughlin | Hill | Thomason | Hester | 3:30.84 | Verified |
| 4 | Blue Springs High School | A | Haupt | Griffin | Brickhouse | Jantzen | 3:22.63 | Verified |
| 5 | Rock Bridge High School | A | Hood | Allen | Seamon | Giles | 3:25.63 | Verified |
| 6 | Blue Springs South High School | A | Townsend | Stokes | Howard | Dowler | 3:31.49 | Verified |
| 7 | Grain Valley High School | A | Allen | Weems | Hart | Mccain | 3:33.61 | Verified |
| 8 | Troy Buchanan High School | A | Benhardt | Carr | Ryan | Lollar | 3:40.12 | Verified |
