| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HEMMER, Audrey | SO | Troy Buchanan High School | 1:09.69 | Verified |
| 3 | UTLEY, Sheridan | SO | Timberland High School | 1:07.21 | Verified |
| 4 | HOLDCROFT, Sadie | 9 | Blue Springs South High School | 1:04.87 | Verified |
| 5 | FREEMAN, Gretchen | SO | Blue Springs South High School | 1:05.48 | Verified |
| 6 | DOMEIER, Isabelle | 9 | Hickman High School | 1:08.00 | Verified |
| 7 | SCHULTZ, Brianna | JR | Battle High School | 1:12.53 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MILLER, Lauren | 9 | Rock Bridge High School | 1:02.91 | Verified |
| 2 | STEMMONS, Sy'Rae | 9 | Hickman High School | 1:02.10 | Verified |
| 3 | HUFFMAN, Avery | SR | Grain Valley High School | 59.11 | Verified |
| 4 | STUART, Paige | 9 | Blue Springs High School | 57.80 | Verified |
| 5 | JACKSON, Ariana | SO | Blue Springs High School | 58.74 | Verified |
| 6 | PARSONS, Grace | JR | Rock Bridge High School | 1:01.15 | Verified |
| 7 | BUSATERI, Breanna | SR | Timberland High School | 1:02.29 | Verified |
| 8 | ANYE, Sarah | JR | Battle High School | 1:04.57 | Verified |
