| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Grain Valley High School | A | Fisher | Parker | Rogers | Torres | 51.58 | Verified |
| 2 | Hickman High School | A | Smith | Leach | Zwingle | Hoffman | 51.03 | Verified |
| 3 | Rock Bridge High School | A | Porter | Crane | Gerrish | Nunnelly | 49.76 | Verified |
| 4 | Blue Springs South High School | A | Brown | Ross | Mason | Sartain | 49.19 | Verified |
| 5 | Blue Springs High School | A | Calloway | Dixon | Hughes | Jenkins | 49.25 | Verified |
| 6 | Timberland High School | A | Adams | Tucker | Adams | Lightbody | 50.75 | Verified |
| 7 | Battle High School | A | Carter | Weitkemper | Brookins | Schultz | 51.45 | Verified |
| 8 | Troy Buchanan High School | A | Crouch | Hunolt | Robinson | Starkey | 51.59 | Verified |
