| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hickman High School | A | Holt | Zink | Milo | Wright | 44.99 | Verified |
| 2 | Timberland High School | A | Schaefer | Hester | Whitehead | Ervin | 44.48 | Verified |
| 3 | Blue Springs South High School | A | VanNatta | Patrick | Watson | Reeves | 43.29 | Verified |
| 4 | Rock Bridge High School | A | Seamon | Giles | Johnson | Mcdaniel Jr | 43.11 | Verified |
| 5 | Blue Springs High School | A | Haupt | Gray | Walton | Olah | 43.19 | Verified |
| 6 | Troy Buchanan High School | A | Giacopelli | Benhardt | Matech-Edwards | Bova | 43.87 | Verified |
| 7 | Grain Valley High School | A | Jones | Pouncil | Weems | Larson | 44.48 | Verified |
| 8 | Battle High School | A | Holt | Ojo | Rice | Owusu | 45.38 | Verified |
