| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Troy Buchanan High School | A | Crouch | Hunolt | Robinson | Starkey | 1:50.78 | Verified |
| 2 | Grain Valley High School | A | Huffman | Rogers | Fisher | Ogle | 1:47.34 | Verified |
| 3 | Blue Springs South High School | A | Brown | Ross | Sartain | Mason | 1:44.07 | Verified |
| 4 | Blue Springs High School | A | Dixon | Calloway | Jackson | Jenkins | 1:41.20 | Verified |
| 5 | Rock Bridge High School | A | Porter | Crane | Gerrish | Nunnelly | 1:43.53 | Verified |
| 6 | Hickman High School | A | Orr | Smith | Peterson | Leigh | 1:46.72 | Verified |
| 7 | Timberland High School | A | Adams | Busateri | Utley | Tucker | 1:49.53 | Verified |
| 8 | Battle High School | A | Weitkemper | Butler | Brookins | Anye | 1:51.03 | Verified |
