| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Fox High School | A | Nguyen | Gast | Kirkpatrick | Pisoni | 1:38.32 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jackson High School | A | STRICKLAND | Settle | DRUM | COFFEY | 1:36.90 | Verified |
| 2 | Mehlville High School | A | Jones | Clayton | DUBOSE | OHMER | 1:35.00 | Verified |
| 3 | Webster Groves High School | A | Logan | Miller | Lewis | Casseus | 1:34.13 | Verified |
| 4 | Poplar Bluff High School | A | McBride | mitchell | Gatewood | Hoss | 1:31.08 | Verified |
| 5 | St. Louis University High School | A | Harris | Oliphant | Bailey | Wingo | 1:31.24 | Verified |
| 6 | Seckman High School | A | Gossett | Harp | Tilley | Rudanovich | 1:34.85 | Verified |
| 7 | Lindbergh High School | A | Balbo | Earley | Goss | Hertel | 1:35.46 | Verified |
| 8 | Oakville High School | A | Williams | Abuzaid | ALTENBERND | MUSTAJBEGOVIC | 1:37.27 | Verified |
